Androgenetic alopecia (AGA) is a polygenetic hair loss disease that causes terminal hair transformation into vellus hair with the progressive miniaturization of the hair follicle. Androgen hormone interaction, imbalance of signaling modulation, genetics, aging, and microinflammation are complex contributions to the pathogenesis of AGA. In the early stage of disease, the peripilar sign represents the sign of microinflammation by showing the infiltration of perifollicular inflammatory cell clusters, including lymphocytes, a few mastocytes, and predominantly macrophages. This study aims to identify the hair follicle macrophage in AGA and comprehensively compare macrophages at various hair follicle compartments. The immunohistochemistry CD68 macrophage markers were stained in 2 healthy and 3 AGA with peripilar signs scalp biopsies. The CD68 positive cell infiltration was not significantly different in each hair follicle compartment of both groups. Furthermore, the infundibulum area seems to have the highest number of CD68 positive cells infiltration, significantly different from the supra-bulb regions of the AGA group and particularly from the bulge and supra-bulb area in the normal group. This study eventually elucidated the immunohistochemical staining for the systematic profile of hair follicle macrophages in normal and AGA hair follicles.