RSUSSH 2020
IN20-246 A Preliminary: Effect of Use of Mouth Rinse Containing Dextranase and Nisin on Oral Plaque in Volunteers
Presenter: Nucharee Juntarachot
Chiang Mai University, Thailand
Abstract
Dental plaque is formed on the tooth surface by oral microbial, namely pathogens bacteria that cause oral diseases such as gingivitis and dental caries. Oral plaque structure is rich in dextran. The mechanisms for preventing oral diseases are the elimination of pathogenic microbes and plaque removal. Further investigation on the prevention has led to the idea of creating a mouthwash that contains important substances which reduce pathogens and plaque in the mouth, such as nisin and dextranase. The dextran-hydrolyzing enzyme or dextranase hydrolases the dextran. Besides, the nisin is a bacteriocin produced from lactic acid bacteria that can be used as a food additive and preservative to inhibit microbial growth such as oral pathogens. The preliminary experiment is reporting the effects of using the mouth rinse containing 2% dextranase and 0.02% nisin (MWDN) on the dental plaque in volunteers. The plaque index was decreased after the use of MWDN for one month. The plaque index was 2.317 ± 0.17 and 1.757 ± 0.16 at the baseline and after the treatment, respectively. Lastly, the MWDN was evaluated through a sensory test in 10 volunteers. The acceptability was used as a 5-point hedonic scale. Most volunteers agreed at Level 3 (like slightly). In the future, the results of this study can be a reference for the formulation development of MWDN. The researchers were funded by the Thailand Research Fund, Research and Researcher for Industry (RRI) and Supporting Research Funds for Industries (SURF).
Citation format:
Juntarachot, N., Tongpong, P., Sittiprapaporn, P., Sirilun, S., Kantachote, D., Bhagavathi Sundaram, S., & Chaiyasut, C.. (2020). A Preliminary: Effect of Use of Mouth Rinse Containing Dextranase and Nisin on Oral Plaque in Volunteers. Proceeding in RSU International Research Conference, May 1, 2020. Pathum Thani, Thailand.QUESTIONS & ANSWERS
Before and after the experiment plaque score index decrease significantly. Sensory test at level 3. Maybe we can do mouth rinse as commercial products for reduce plaque in the future as well.
Before and after the experiment plaque score index decrease significantly. Sensory test at level 3. Maybe we can do mouth rinse as commercial products for reduce plaque in the future as well.
Do you have control in this study? Did you remove plaque before gave the mouthrinse to volunteer to use? Is this mouthrinse aim for regular mouthrinse for everyday use? This is interesting to be explored more in future.
Dear Assoc. Prof. Thanasit Sererat
Thank you very much for the review of our manuscript. We very much appreciated your encouraging and insightful comments.
Dear อ.ทญ.จณัญญา เปลี่ยนรัังษี
We greatly appreciate your thoughtful comments that helped improve the experiments. Thank you very much for your effort. In the following, we give a point-by-point reply to your comments:
The experiment does not have control (treatment control) in this study because we would like to study the efficiency of the mouth rinse to plaque change before and after.
We recommended using the mouth rinse after lunch and dinner every day, because of the aim of the mouth rinse is easy to use that Instead of brushing teeth during the day. However, the efficiency to protect the oral cavity and sensory acceptability are very important too.
Thank you for your nice presentation. Apart from reduction in plaque after using the mouthrinse do you observe any changes or improvement in gingival conditions? Do you give any oral hygeine instructions to the volunteers before the experiment?
Dear PROMPHAKKON KULTHANAAMONDHITA
Thank you for your interest in our research. The result found that all of the volunteers did not have the problem of gingivitis before the experiment so, it not change significantly for improvement in gingival conditions after the experiment. However, thank you for your good question that is a good idea for the research.
The next answer is no. We just recommended using the mouth rinse before the experiment to control the volume of the mouth rinse because we do not need to change the behavior of the volunteer. In addition, we would like to check the efficiency of the mouth rinse.
Thank you for your nice presentation. I have a question for you as:
How you have studied that your mouth rinse preparation ingredients are compatible with active ingredient.
Dear DR. NRIPENDRA SINGH
Thank you for your question. The active ingredients can dissolve with the mouth rinse preparation ingredients that showed the homogenized characteristic. Moreover, we studied the antibiofilm activity (In-vitro) between the mouth rinse containing active ingredients and the mouth rinse base. The result found that the mouth rinse containing active ingredients can reduce the biofilm more than the mouth rinse base.