The imbalance of increased energy consumption has occurred while the fossil fuel resource has been decreasing. Therefore, finding of renewable energy is important and challenging for our World energy. The third generation of bioresources comes from cyanobacteria that can produce bioenergy compounds, such as ethanol, alkanes and fatty alcohol, via their atmospheric CO₂ fixation. However, the main limited productivity is cell and membrane damages by free radicals-generating alcohol products. In this study, one of tolerant mechanisms to alcohol stress is focused on phospholipid modification. We aimed to monitor alcohol tolerance level in cyanobacterium Synechocystis sp. PCC 6803 with overexpressing plsX (slr1510) gene, encoding putative acyltransferase involved in phospholipid synthesis (hereafter OX_plsX). Cell growth of OX_plsX was higher than WT and control WT under both normal and alcohol stressed conditions, as well as their oxygen evolution rates. The cell growth recovery of OX_plsX showed highest level at day 3 to day 5 of recovery duration after 24h-treatment of 8% (v/v) ethanol. Also, the color of OX_plsX cell culture showed darkest green when compared to other strains. Interestingly, the morphology of OX_plsX was least aggregated under alcohol treatment when compared with WT and control WT. Then, our findings suggested that OX_plsX strain had higher tolerance level to alcohol stress, as well as its growth recovery from alcohol treatment, especially at 8% (v/v) ethanol concentration.