Sadao din (Glinus oppositifolius) pertained to the Molluginaceae family is an herb and widely distributed almost all over Thailand. These plants had been used in local medicine for the treatment of many ailments such as diarrhea, fever and malaria. In this work, the aerial parts of the plant G. oppositifolius was prepared into powder. The powder was extracted with various solvents viz water, ethanol, ethyl acetate and chloroform. Then, the extracts were evaporated for analysis of phytochemicals, total phenolic content (TPC) and antibacterial activities. The phytochemical screening showed that alkaloids and flavonoids were presented in all of the extracts, whereas saponins and tannins were found in aqueous and ethanol extracts. The amount of TPC varied in different extract, moreover, ethanol extract showed the maximum TPC of 142.22±12.51 mg of GAE/g. Furthermore, the extracts were tested the in vitro antibacterial activity against seven bacteria namely Bacillus cereus, B. subtilis, Enterobecter aerogenes, Escherichia coli, Pseudomonas aeruginosaj, Staphylococcus aureus and S. epidermidis using disc diffusion technique. The result showed that B. cereus and B. subtilis were inhibited at the concentration of 2 mg/disc of ethyl acetate extract. The study provided a scientific basis for using of the plant extracts in the retaining of microbial-induced aliments.