The aim of this in vitro study was to evaluate the effect on microshear bond strength of various types of universal adhesive with dual cure resin cement and different types of hybrid ceramic bonded to dentin. Flat buccal surface of 99 extracted human premolars were randomly allocated into 9 groups. Three hybrid ceramics, Shofu block HC^® (SHOFU, Japan), Vita Enamic^® (VITA, Germany) and Cerasmart^® (GC, USA), were prepared into an approximate size of 1 x 1 x 3 mm³ slab and bonded to dentin using Scotchbond Universal^® (3M ESPE, USA) with RelyX™Ultimate^® (3M ESPE, USA), Optibond XTR^® (Kerr, USA) with Nexus3^® (Kerr, USA) and Optibond FL^® (Kerr, USA) with Nexus3^® (Kerr, USA). After 10,000 cycles of thermocycling, all specimens were tested for microshear bond strength. Data were analyzed using a one-way ANOVA and Tukey’s test (α = 0.05). The microshear bond strength in etch and rinse groups, Optibond FL^® with Nexus3^® and Scotchbond Universal^® with RelyX™Ultimate^®, was greater than the self-etch system, Optibond XTR^® with Nexus3^® in all hybrid ceramic tested. Within the limit of the study, there was significant difference between the microshear bond strength when using various types of universal adhesive and resin cement systems with different types of hybrid ceramic. Vita Enamic^® was recommended to cement with Optibond FL^® and Nexus3^®, whereas, Scotchbond Universal^® and RelyX^TMUltimate^® were suggested for Shofu block HC^® and Cerasmart^®.