RSUSSH 2020

IN20-135 microRNA profiles associated with continuous growth of lower incisor in rat model

Presenter: Sirawit Nipakasem
Chulalongkorn University, Thailand

Abstract

          Rodent incisor is an organ that continuously grows throughout its lifetime to compensate for abrasion. Development process of incisors is the result of delayed root formation and prolonged crown formation possibly made by stem cell niche, so-called cervical loop, which involve various genes and  ignaling pathways. However, the growth of molar discontinued after complete root formation. MicroRNAs (miRNAs) are small non-coding single-stranded RNAs that involved in biological mechanisms of dental development. To study the role of miRNA, we extracted total RNA from the dental pulp tissue collected from the apical tips of incisors and molars, and compared the expression of miRNAs. The customized RT-PCR array (Qiagen) demonstrated that the apical part of incisors expressed miR-720 4- fold higher than that from molar. In addition, miR-652-33p in incisors was expressed 2-fold higher than that in molar. In contrast, miR-338-3p and miR-27a-3p was decreased compare to molar group. The results suggested that these miRNAs may have a role in mineralization process during prolonged crown formation of rodent incisors.

Keywords: Rodent incisor; Hypselodont; Dentine formation; Continuous growth; microRNA; Cervical loop; Dental Pulp Stem cell

Citation format:

Nipakasem, S., Sukarawan, W., & Vacharaksa, A.. (2020). microRNA profiles associated with continuous growth of lower incisor in rat model. Proceeding in RSU International Research Conference, May 1, 2020. Pathum Thani, Thailand.

QUESTIONS & ANSWERS

Tadsanee Punjanon (Visitor)

Results for this study suggested that these miRNA may have a role in the mineralization process during prolonged crown formation of rodent incisors.  What is the application of this finding from animals to a human?

Sirawit Nipakasem (Presenter)

Thanks for your question.

 

According to our result, potential of these miRNAs may be a novel material for hard tissue regeneration such as pulp capping material. However, further studies are needed.

 

Anek Chayasadom (Visitor)

Thank you for your interesting presentation. I would like to ask about method of statistical analysis you used in this study.

Sirawit Nipakasem (Presenter)

Thanks for your question

In our study are using descriptive statistics study (Means of the relative expression) due to lack of samples. For the further study, acquiring more samples would validate the significance of the study.