RSUSSH 2020
IN20-288 The use of modified mRNA encoding platelet-derived growth factor-BB as an innovation in periodontal regeneration
Presenter: Theeraphat Surisaeng
Chulalongkorn University, Thailand
Abstract
Since mRNA platform has been recently introduced to several fields in medicine and the achievement of periodontal regeneration is currently unpredictable, the use of mRNA technology tends to be a promising approach to pursue the complete periodontal regeneration. The aims of this study are to learn if mRNA encoding platelet-derived growth factor-BB (PDGF-BB) induces PDGF production in human periodontal ligament cells (PDLCs) as well as to investigate the effect of secreted PDGF on PDLCs proliferation. PDLCs were obtained from extracted teeth of healthy periodontal patients. The modified N1-methylpseudouridine mRNA encoding PDGF-BB were transfected into PDLCs. The supernatants were collected from 24-, 48- and 72-h timepoints and measured the protein production using ELISA. The viability of transfected PDLCs was also assessed. In addition, the supernatants collected at the 24-h timepoint were used for the proliferation assay using AlamarBlue. The result showed that PDLCs, transfected with mRNA encoding PDGF-BB, produced higher level of intracellular PDGF-BB than controls at 24 h. PDGF-BB was also detected in the supernatants started from 24 h and constantly secreted up to 72 h. The transfection of mRNA encoding PDGF-BB had no effect on PDLCs viability. The supernatants containing PDGF-BB were able to promote PDLCs proliferation. Thus, this mRNA platform technology is possibly applicable for periodontal tissue regeneration.
Citation format:
Surisaeng, T., Mahanonda, R., Sa-Ard-Iam, N., Rerkyen, P., Chanamuangkon, T., & Chanpaiboon, C.. (2020). The use of modified mRNA encoding platelet-derived growth factor-BB as an innovation in periodontal regeneration. Proceeding in RSU International Research Conference, May 1, 2020. Pathum Thani, Thailand.QUESTIONS & ANSWERS
Thank you for your nice presentation. The study is very interesting and very up to date.
1. Are there any other studies that use chemically modified mRNA technology for medical tissue regenerartion? Are their results similar or different compare to your study?
2. For clinical uses, what do you think will be the appropriate carrier to apply mRNA into the periodontal defect?
Thank you, Dr. PROMPHAKKON KULTHANAAMONDHITA
1. Some studies use modified mRNA technology for medical regeneration. For example, an experiment of mRNA specific to VEGF-A growth factor in mice with ischemic heart disease demonstrated the successful outcome of using mRNA in tissue regeneration. After receiving the VEGF-A encoded mRNA injection, an increase of new blood vessels was reported. The muscle lesions became smaller and mice lived longer than mice injected with plasmid DNA and the controls (Zangi et al., 2013)
2. Given the delivery of the modified mRNA encoding PDGF-BB transfection, more researches are required to show the proper scaffold or carrier to introduce and also maintain the mRNA solution at a defect site. These additional scaffolds such as controlled bio-absorbable collagen (collagen sponge), collagen membrane, and cell sheets are promising materials.