The objective of this research is to reduce dental anxiety of the patients during local anesthesia injection by dental students. In this pilot study, 60 patients who came into Oral Surgery Department, College of Dental Medicine, Rangsit University during the period between June 2019 to October 2019 were evaluated. Patient data including vital signs (systolic blood pressure, diastolic blood pressure and a heart rate), the anxiety score (MDAS), the pain score (VAS) and the satisfaction score were evaluated by statistical tests. There was no statistically significant difference of vital signs between music and non-music groups (p>0.05). No statistically significant difference of anxiety score between music and non-music groups (p>0.05). The mean of anxiety score preoperatively in music group was 10.66 and decreased to 9.06 postoperatively while the mean of anxiety score preoperatively in non-music group was 10.34 and decreased to 9.58 postoperatively. There was a statistically significant difference of preoperative anxiety score and postoperative anxiety score in music group (p<0.05) while there was no difference in non-music group (p>0.05). The use of music therapy is a simple, safe, inexpensive and non-pharmacological intervention that can effectively reduce dental anxiety of the patients during local anesthesia injection.