Cancer has become the second most common cause of death, and its incidence and mortality rates have increased rapidly worldwide, including Thailand. Despite several types of cancer treatment being available, these treatments are expensive and associated with terrible side effects and eventual resistance. Plant-derived natural products have been developed as anti-cancer drugs because of their safety and efficacy. Several plants in the family Zingiberaceae have been reported to possess anti-cancer properties against several types of cancer. This study sought to determine the potential anti-proliferative activity of 9crude extracts from Zingiberaceae plants towards human embryonic kidney (HEK 293T/17) cells. The extracts were Zingiber officinale Roscoe (CE1), Alpinia galanga (L.) Willd (CE2), Curcuma mangga Valeton & Zijp (CE3), Curcuma aeruginosa Roxb. (CE4), Curcuma longa L. (CE5), Boesenbergia rotunda (L.) Mansf. (CE6), Kaempferia parviflora Wallich. ex Baker. (CE7), Zingiber montanum (J.Koenig) Link ex A.Dietr. (CE8), and Zingiber ottensii Valeton (CE9). The cytotoxic effects of crude extracts were determined by MTT assay. The results showed that all nine crude extracts had a strong cytotoxic effect on HEK 293T/17 cells in a concentration-dependent manner. The 50% cytotoxic concentration (CC₅₀) of CE1-CE9 were 0.228, 0.235, 0.261, 0.230, 0.187, 0.160, 0.190, 0.218, and 0.266 mg/ml, respectively. The most significant cytotoxic effects were seen with CE5 and CE6. These two crude extracts had the lowest CC₅₀ and extremely affected morphology of treated cells at low concentrations, suggesting that CE5 and CE6 have the potential to be the candidates for cancer therapeutic agents.