Clinacanthus nutans (Burm.f.) Lindau belongs to the Thai national list of essential medicine (NLEM). This plant has been used in the treatment and prevention of a variety of symptoms such as anti-viral, anti-bacterial, and anti-inflammatory activity. In this study, we investigated the effects of different extract fractions of C. nutans on anti-cell death. Firstly, the C. nutans fractionation was performed by sequential extraction using different organic solvents including hexane, dichloromethane, ethyl acetate, and water, respectively. We further investigated its anti-apoptosis activity in bovine endothelial cells (CPAE) after the treatment of Escherichia coli lipopolysaccharide (LPS) by using PrestoBlue™ cell-viability assay. The result showed that LPS strongly induced cell deaths with the CC50 values of LPS of 57.62 ng/mL. Interestingly, all fractions of C. nutans extract significantly lower the LPS-induced cell death in a dose-dependent manner. The ethyl acetate fraction was the most effective to protect cell death. At 125 μg/mL, it could protect more than 80% of the cells. Furthermore, the bioactive compound glyceryl 1,3 distearate (C₃₉H₇₆O₅) was measured in C. nutans extract by using TLC analysis which could be the major compound that contributed to the anti-apoptosis activity. Our finding could lead to the development of C. nutans extract to treat inflammation during bovine mastitis.