The number of teeth remaining in an individual older relative to the increased risk of frailty and mortality. However, biological aging significantly affects the regenerative function of human periodontal ligament cells (hPDLC), leading to a marked decrease in osteogenic differentiation and mineralization. Asiaticoside was proven to affect the regenerative ability of hPDLCs. Therefore, this study aimed to determine the effect of asiaticoside on aging hPDLCs osteogenic gene expression and bone mineralization. The cells were retrieved from patients aged 60 and above and treated with asiaticoside at various concentrations to evaluate the percentage of cell viability using the MTT assay. Various osteogenic genes were analyzed using real-time polymerase chain reaction (PCR). Mineralization on day 14 was determined using alizarin red staining. The results show that asiaticoside had no toxicity on aging hPDLCs at concentrations of 25 µM and below. The expression of RUNX2, OSX, and BMP9 was significantly upregulated after treatment with asiaticoside for 24 hours (P<0.05). Interestingly, the expression of COL1 was remarkably decreased, while BMP2 and DMP1 were upregulated and mineralization nodules were significantly enhanced in aging hPDLCs. This study suggested that asiaticoside has the potential to be used in periodontal regenerative treatment for elderly patients.