This prospective pilot study focused on the relative occlusal forces of implant prostheses in differently designed implant occlusion. It aimed to analyze the relative occlusal forces of single posterior implant-supported fixed prostheses over time by the T-scan system and compare the marginal bone loss of single posterior implant-supported fixed prostheses over time. Twelve patients who needed a single posterior implant-supported prosthesis were randomly divided into two groups which were Implant-protected occlusion (IPO) and LOAD groups. All patients were received with implant treatment protocol according to the faculty standard. However, the implants in the IPO group presented with occlusal contact at the heavy bite and no occlusal contact at the light bite. While for the LOAD group, implants exhibited occlusal contact at the heavy bite and light occlusal contact at the light bite. The relative occlusal forces were measured using T-scan at five different times, before implant crown delivery, after implant crown delivery, after function 1-2 months, 3-4 months, and 6-7 months. The periapical parallel x-rays were taken using a customized jig to analyze marginal bone loss before implant crown delivery, after implant crown delivery, and after function 6-7 months. The result showed no significant difference of the relative occlusal forces between the IPO and LOAD groups. The IPO group showed no significant difference in relative occlusal force between implant restoration and contralateral natural tooth after function 3-4 months. While in the LOAD group, the relative occlusal force between implant restoration and contralateral natural tooth after function 1-2 months was not significantly different. However, both groups showed that the relative occlusal force of implant restorations increased over time. Therefore, there was no significant difference between IPO and LOAD groups for marginal bone loss. The LOAD occlusion that lets the implant occlude faster seems convincing since the marginal bone loss did not show any significant differences. However, the finding was inconclusive whether LOAD occlusion is better than IPO. A thorough follow-up study and increased sample size are needed.