This is the study in vitro compared the shear bond strength between acrylic resin cement or self-adhesive resin cement with and without prior universal adhesive application to human dentin. Thirty-five extracted permanent third molars were collected and embedded in self-cured acrylic molds exposing the buccal surface of superficial dentin. Resin composite cylinders (Filtek Z350XT) with 2.38 mm diameter were prepared and cemented using the following combination of resin cement and universal adhesives; group 1: Rely X U200 (RU), group 2: Single Bond Universal Adhesive and Rely X U200 (SBU-RU), group 3: Panavia SA Luting Multi (PSA), group 4: Clearfil Tri-S Bond Universal Quick and Panavia SA Luting Multi (CBQ-PSA), and group 5 Super Bond C&B (SB). All the specimens were then stored in an incubator at 37^oC and subjected to 5,000 cycles of thermocycling. After that, the shear bond strength test was evaluated using the Universal testing machine (EZ-S, SHIMADZU). One-way ANOVA and Tukey’s post hoc test were used to statistically analyze the shear bond strength values. SBU-RU had the highest shear bond strength, followed by SB which was higher than the remaining groups. The shear bond strength of RU was not different from CBQ-PSA but greater than PSA. However, the shear bond strength of CBQ-PSA was not different from PSA. Overall, applying universal adhesives before self-adhesive resin cement showed significantly higher shear bond strength only when applied with Rely X U200 (p < 0.05) and Rely X U200 demonstrated higher bond strength than Panavia SA Luting Multi (p < 0.05). In Conclusion, Super Bond C&B had the highest shear bond strength compared to non-universal adhesive-coating self-adhesive resin cement. However, with universal adhesive coating, Single Bond Universal Adhesive and Rely X U200 combined yielded the highest shear bond strength.