Now a day, the novel dynamic navigation system (DNS) was shown to improve the correct 3D position of implant placement in anterior teeth, but quite difficult to gain proficiency. Even though the distributed training (lengthy rest periods) was proved to be better than the massed training (long training with short break), but it is still unclear whether differences in rest periods of distributed training will gain the best motor skill of dynamic navigation system. Thus, the objective of this study was to evaluate the most efficient training program with variable rest periods to achieve motor skill acquired with the highest level of accuracy in implant placement using DNS. Twenty senior dental students with no experience in implant placement and DNS were randomly and equally assigned into a group of the distributed training programs that lasts many days (group A) and distributed training that is done in one day (group B). All participants placed three implants in a training session and one implant after 7 days of third repetition as a post-test. The accuracy was measured from 3D deviation at implant platform, implant apex, and angle deviation. The independent T-test and Mann-Whitney test were used to determine differences between groups, and the repeated ANOVA test and Friedman-Dunn test were used to determine differences within groups, which 0.05 was the significant level. There were no significant differences in improvement of accuracy between the groups during all repetition and post-test. The training program that lasts many days and is done in one day didn’t cause any significant improvement of motor skill acquisition of implant placement by using the dynamic navigation system