This study aims to evaluate the surface roughness of heat-cured acrylic resin after immersion in 4 different solutions of household agents and a commercial denture cleansing solution. Seventy-two 10x10x2 mm³ disc-shaped specimens were fabricated from heat-cured acrylic resin and divided into 6 groups. Twelve specimens in each group were immersed in 4 household agents (100% clear vinegar, 5% acetic acid, and 0.1% and 0.5%  Sodium hypochlorite), while a commercial denture cleansing solutions: Polident^® (Block Drug Company Inc, Memphis, TN38113, USA.) and tap water served as a negative control. All specimens were immersed 10 minutes/time, 5 times/day for 36 days representing 6 months of clinical service. The surface roughness (Ra, μm) was measured before and after immersion with a non-contact surface roughness tester (Infinite Focus SL, Alicona^®, Austria). All data were compared by One-Way ANOVA and Tukey test, with a significance level of 0.05. The mean difference of surface roughness of the control group and test group; Polident^®, 100% clear vinegar, and 5% acetic acid, was not significantly different (P > 0.05) but 0.1% and 0.5% sodium hypochlorite showed a significant difference (P < 0.05) in higher surface roughness. The surface roughness of heat-cured acrylic resin immersed in 0.1% and 0.5% sodium hypochlorite was significantly different from the other groups. 100% clear vinegar and 5% acetic acid were not significantly different from Polident^®, which is the representative of commercial denture cleansing solutions. Therefore, 100% clear vinegar and 5% acetic acid that are household agents can be the alternative options for the routine used.