The study evaluated the influence of Biodentine, TheraCal LC, and Tideglusib on pulpal morphology and dentin mineralization for direct pulp capping material in the tooth culture model. Thirty-six human immature third molars freshly extract from patients aged 15-19 years were used to study the pulpal reaction after the pulpal medication with different pulp capping materials. Six experimental material groups were determined; three different concentrations of Tideglusib (10, 50, and 100 nM), two commercially pulp capping materials (Biodentine and TheraCal LC), and control as a scaffold (Polycarprolactone) soak with DMSO₄ in DMEM media. The materials’ interactions with the vital pulp were investigated using the tooth culture model for 14 and 28 days (n = 3). The mineralized foci and the mineralization potential were evaluated by histology. The direct pulp capping with Tideglusib showed biocompatibility to dental pulp cells. The positive control Biodentine and TheraCal LC showed the mineralization nodule at 14 and 28 days. The tooth culture model was a valuable technique for biocompatibility tests in dental pulp cells. Biodentine and TheraCal LC have shown a promising mineralize potential. Tideglusib was biocompatible and promote inflammatory healing to pulp cells, however, the mineralized potential was absent.