Addictive-like eating behavior has been studied to understand the underlying mechanisms of food addiction-induced obesity disease. The highly palatable food including chocolate can trigger addiction processing by activating the same drug-generated reward system in the brain. However, the influences of chocolate consumption especially without fasting condition on motivational reward effect in response to conditioned environmental cues have remained a controversy. Therefore, the purpose of this study was to determine the reward effect of chocolate-induced condition place preference (CPP) in comparison with the standard addictive drug, morphine. Male Swiss albino ICR mice (20-30 g) were used to examine reward-associated cues using a Y-shape CPP apparatus. The 27-day experiment consisted of habituation, pre-condition, condition, post-condition, extinction, extinction-test, and reinstatement periods. The condition induced-behaviors (e.g., CPP score, number of entries, locomotor) were analyzed to determine the motivational reward. The authors found that the sated animal did not reveal significant changes of chocolate condition induced-behaviors during all 3 conditions: post-condition, extinction-test, and reinstatement periods. In contrast, morphine treatment induced obvious changes in behavioral patterns associated with morphine condition including CPP score and the number of entries. In conclusion, these results indicated that chocolate cues might not be strong enough to trigger processing in the brain of sated mice.