Silver nanoparticles (AgNps) are incorporated in a variety of medical applications due to their antimicrobial abilities. Green synthesis of AgNps is relatively new, and the investigation of this phenomenon has acquired attention lately as there is an increase in bacterial resistance towards antibiotics. Hence, the purpose of this research was to determine the antimicrobial activities of AgNps synthesized through the green synthesis method using spent coffee ground (SCG). First, AgNps were generated by mixing hydro-alcoholic SCG extract with silver nitrate solution. The AgNps were characterized using Malvern Zetasizer analyzer for size determination. Antibacterial potentials of AgNps produced were elucidated using both disc diffusion and broth dilution assays. The nanoparticle sizes obtained exhibited unimodal distribution that centered around 164-190 nm. Disc diffusion assay showed that the AgNps (0.144mg) were able to display broad-spectrum inhibition against Gram-negative Urinary Tract Infections (UTIs)-causing pathogens: Escherichia coli (zone of inhibition = 10.17 ± 0.58 mm) > Pseudomonas aeruginosa (zone of inhibition = 10 ± 0.71mm) > Proteus mirabilis (zone of inhibition = 7.33 ± 0.62 mm) > Klebsiella pneumoniae (zone of inhibition = 7.25 ± 0.75 mm) (according to size of inhibition zone). However, in broth dilution, K. pneumoniae (MIC = 200µg/ml) demonstrated the highest susceptibility towards the synthesized silver nanoparticles, while P. mirabillis (MIC = 500µg/ml) was the least susceptible. In conclusion, silver nanoparticles generated by the green synthesis method using spent coffee ground are promising antibacterial agents against the tested UTI-causing bacteria, and this warrants further investigation.