Experiments using different kinds of rat feed diet is being used in metabolic studies. Research using different diets on Sprague Dawley rats to see their effects on their physiology are well documented. Our aim is to identify the diet which promotes the best body weight and blood glucose control.  Thirty-five Sprague Dawley rats were subdivided into five categories and were given five different diets for eight weeks. Namely the five diets are normal rat chow, high sugar, high starch, high protein (whey-based) and high fat rat feed formula. The rats were given specified diet pellets ad libitum with tap water. The result showed high protein diet significantly promotes body weight control and moderate blood sugar control among all the diet given. In conclusion, the preference of a high protein diet is commendable to ameliorate obesity and its negative effects, such as metabolic syndrome.