Coronavirus disease 2019 (COVID-19) became a pandemic in March 2020. Following this pandemic, people realized the importance of hand hygiene as one of the effective ways to prevent disease transmission. This study aims to measure the knowledge towards hand hygiene among the general population during this pandemic period. A cross-sectional online survey study was conducted from August to September 2020. The questionnaire that was mainly based on the WHO hand hygiene reference consisted of 2 parts; 12 items in demographic data and 10 items in the knowledge of hand hygiene. A total of 200 participants completed the questionnaire. More than half of the participants (61%) exhibited a “good” level of knowledge, whereas some participants (5%) had a “poor” level of knowledge. Moreover, many participants did not know the sufficient time duration required for effective hand rubbing and handwashing with soap and water. Approximately half of the non-healthcare providers did not recognize the association between frequent handwashing and incidence of hand eczema. This study demonstrated that most participants had good knowledge of hand hygiene for disease prevention. However, they still had a misconception of the appropriate time required for different types of handwashing. Education on proper hand hygiene is necessary to improve people’s knowledge, especially in non-healthcare providers. Prospective studies with more participants and generalizability are required to assess the gap in people’s knowledge and prepare for the next wave of the pandemic.