Nowadays, studies of the roles of microRNAs (miRNAs) in hepatocellular carcinoma (HCC) have either focus on specific individual miRNA and a few suspected targets or simply reported a list of potential miRNAs based on expression profiling. In this study, we investigated a more in-depth understanding of the roles of miRNAs and their targets by using bioinformatic tools for screening miRNAs as potential biomarkers to early diagnostic of HCC. Datasets were collected from Gene Expression Omnibus (GEO database) by keyword: “human hepatocellular carcinoma microRNA”. There were four datasets selected including GSE62044 (combine GSE62007 and GSE62043), GSE74618, GSE21362, GSE10694. We assessed the level of mature miRNAs in all of the above with more than 1000 miRNAs and 937 HCC samples of patients in many countries. Statistical analysis indicates that twelve of these miRNAs, which present in all of the datasets, have significant alteration in the expression level. Seven of these miRNAs (miRNA-221, miRNA-222, miRNA- 224, miRNA-93, miRNA-25, miRNA-106b, miRNA-155) were up regulated in HCC and the other five miRNAs (miRNA-34a, miRNA-99a, miRNA-139-5p, miRNA-422a và miRNA-214) were down regulated. All of them have relevance to the hallmarks of cancer such as cell proliferation, differentiation, invasion, metastasis, angiogenesis, metabolism and immune system. In HCC patients, the portion of surviving and month surviving in query genes, which have modified, are lower than they have not modified. These were evidence supported for the correlation between levels of miRNA expression and HCC, therefore, they may be considered as biomarkers for early diagnosis in HCC. Furthermore, this could be a list of potential miRNAs to carry out further dept-studies in wet-laboratory.