- Where is your slides presentation? This part is very important for the oral presentation. You are lack of the slide so almost audiences can not catch up your details or your story. It make your presentation lack of interesting.

- Why did the author use 0.2 %vitexin ?
- The author should vary the concentration of vitexin.
- How did the author measure the area of hair regrow this?
- How did the author treat the vitexin ?

- The author mentioned that vitexin was effective in in vitro study but opposite results in in vivo study.
But the reasons that the author give quite confuse. So I would like you to give more reasons and explain in the facile description.

Vitexin is a natural product.  How is the stability of vitexin?

Thank you for your questions and suggestions, ผศ.ดร.ปานันท์ กาญจนภูมิ (CHAIRPERSON)

- For slides, I'm really sorry for the technique that I cannot put the slide together with my video.

- Why did the author use 0.2 %vitexin ?

        For concentration of topical solution of vitexin, it was calculated by using the concentration of vitexin that showed anti-inflammatory effects, one of important pathogenesis of AGA, in previous cell and animal studies because of no previous in vivo study of hair growth- promoting effect of vitexin and no topical vitexin used before. From reviewing of previous studies, the doses of vitexin used for mice and rats studies are oral administration 5, 15 and 30 mg/kg and intraperitoneal injection 10 mg/kg. And the concentrations used in cell studies are 50 and 100 μg/ml of vitexin and 25 μM (= 10.8 μg/ml) of vitexin. From these evidences, the doses and concentrations of vitexin used are quite vary in wide range. So that, from all the knowledges the author collected, the lowest concentration of vitexin (25 μM or 10.8 μg/ml) is used to calculate the concentration of topical solution of vitexin. The concentration of 10.8 μg/ml is equal to 0.001 gm/100 ml. The skin penetration coefficiency is about 1:200. So, the concentration of topical solution of vitexin is 0.2%w/v (0.2 gm of vitexin in 100 ml of 60% ethanol).

- The author should vary the concentration of vitexin.

        The author decided to use the lowest concentration as mentioned above and it would save experimental cost. By the way, thank you for the comment, the author also interested in this point and might include it in the next study.

- How did the author measure the area of hair regrow this?

        Photographic data was analyzed by using Adobe Photoshop 6.0 software. The area of hair regrowth was calculated by using the dark or black area (represent the hair regrowth area) divide by the total area of hair clipped area.

- How did the author treat the vitexin ?

        0.2% vitexin was topically applied 0.1 ml/clipped area once daily for 21 days.

- The author mentioned that vitexin was effective in in vitro study but opposite results in in vivo study.
But the reasons that the author give quite confuse. So I would like you to give more reasons and explain in the facile description.

        The author already added more explanation in discussion part of the papers. By the way, thank you for the comment.

Thank you for your questions, TADSANEE PUNJANON

- Vitexin is a natural product.  How is the stability of vitexin?

       Vitexin was first used as a topical solution in this research. The stability of vitexin is an important point to concern. The authors also concerned this point. So, first thing, the authors dissolved the vitexin in 60% ethanol which is able to use together. Second thing, the authors did mixed the solution on the period of application.