RSUSSH 2020
IN20-219 Effect of extraction methods on yield, total phenolic content and antioxidant activity of Ipomoea pes-caprea (L.) R. Br. leaves
Presenter: Monsiri Chetkhetkran
Burapha University, Thailand
Abstract
Ipomoea pes-caprea (L.) R. Br. is traditionally used in nutrition, medicine, and agriculture in Thailand to treat inflammation of toxic effects from the jellyfish venoms and dermatitis. However, I. pes-caprae still has a limited study on the selection of suitable extraction techniques for up scaling purposes. The aim of this study is to use different extraction methods: maceration assisted extraction, ultrasound assisted extraction, microwave assisted extraction, and espresso machine for evaluating the yield, total phenolic contents as well as determining antioxidant activity (DPPH and ABTS scavenging) from I. pes-caprae leaves. Each part of the plant was extracted by reflux using 5 different methods such as maceration, microwave, ultrasound, and espresso machine. Total phenolic content was determined with Folin-Ciocalteu 1:10 on 765 nm using a microplate reader. Antioxidant activity was determined using DPPH and ABTS free radical scavenger methods. The yield of I. pes-caprae extracts isolated with different methods showed the highest yield was associated with 50% EtOH maceration extraction (18.60%). Total phenols content and DPPH free radical scavenging activity were the highest value in 50% EtOH with microwave 5 min (64.26±0.33 mgGAE/g and IC50 24.07±0.03 µg/mL respectively). The crude extracts displayed ABTS free radical scavenging activity with the highest value in 25% EtOH espresso machine (IC50 488.71±0.42 µg/mL). This study demonstrates that microwave assisted extraction for 5 minutes with 50% EtOH of pes-caprae leaves showed the most compromising result in terms of the efficient extraction and recommended for a potential source of natural antioxidants and high total phenolic content for commercial uses.
Citation format:
Teerakulkittipong, N., Phosri, S., & Chetkhetkran, M.. (2020). Effect of extraction methods on yield, total phenolic content and antioxidant activity of Ipomoea pes-caprea (L.) R. Br. leaves. Proceeding in RSU International Research Conference, May 1, 2020. Pathum Thani, Thailand.QUESTIONS & ANSWERS
1. Antioxidant activity was determined using DPPH and ABTS free radical scavenger methods. How does the difference happen between ABTS and DPPH radical scavenging activity?
2. What about a standard for DPPH and ABTS assay?
Thank you very much for your nice presentation! In this research, each part of the plant will be extracted by reflux using 5 different methods such as maceration, microwave, ultrasound, and espresso machine. Can you provide more details on physicacochemical properties of extraction from 5 different methods.
Thank you for your questions
ผศ.ดร.อรจิรา อารักษ์สกุลวงศ์ (CHAIRPERSON): - Antioxidant activity of the extracts analyzed and determined by radical scavenging activities and reducing power. DPPH is one of the most widely reported techniques for measuring antioxidant activity. The DPPH assay determines a change in antioxidants that could be polar e.g. phenolics, flavonoids, etc. The ABTS assay uses ABTS radicals performed by oxidation of ABTS with potassium persulphate. Antioxidant compounds and ABTS react to form a radical cation, ABTS+ .
DR. SURACHAI KARNJANAKOM (VISITOR): We used 4 extraction methods such as microwave-assisted, maceration technique, ultrasound-assisted extraction, and espresso machines and test suitable duration time for extraction in which these advances are schemed to increase yield, green environment with high efficacy at a lower cost.
(1) Microwave power approved the solvent’s movement into the cell plant, increasing the extraction efficacy, less extraction time and low temperature.
(2) Maceration technique is commonly used by intending to soften and break the plant’s cell wall to release the soluble high amount of phytochemicals but this process has a long duration time and a large volume of organic waste.
(3) Ultrasound extraction is used to disrupt plant cell walls, which helps improve the solvent’s ability to penetrate the cells and obtain a higher extraction yield.
(4) Espresso machines used the high pressurized and high-temperature solvent extraction system with a short time to separate the active ingredients from plants.