Ingredients form natural product have been used for centuries for skin care purposes. Nowadays, they are becoming more widespread in cosmetic formulations, due to consumers are becoming increasingly concerned about synthetic ingredients substances. The main advantage reported for plant extracts, used in skin care, include antioxidant, and anti-aging. In this study, ten herbal medicines used in Thailand were investigated for anti-hyaluronidase and anti-elastase activities, and their activities were investigated using enzyme-substrate assay. Ethanol extract of Senna garrettiana at the concentration of 0.25 mg/ml had the highest hyaluronidase and elastase inhibitory activity. The inhibitory percentage of S. garrettiana heartwood extract against hyaluronidase and elastase were 60.6 ± 0.8 and 62.5 ± 0.6 %, respectively. The result indicated that S. garrettiana heartwood extract has a potential use on anti-aging skin care products due to their aging-enzyme inhibition properties. The ethanol extract of S. garrettiana heartwood was therefore selected and subjected to isolate of the active anti-hyaluronidase and anti-elastase compounds. On the basis of bioassay-guided isolation, betulinic acid and piceatannol were obtained as an active compound from S. garrettiana heartwood extract, that exhibited satisfactory for anti-elastase activities with IC₅₀ values of 20.5 and 15.2 µg/ml, respectively. With regard to anti-hyaluronidase studies, no activity was observed with isolated compounds at the concentration of 100 µg/ml.