Rice (Oryza sativa) is the most important food for population in the world. Rice contains bioactive compound which shows anti-aging and anti-cancer properties. Therefore, the aims of this study were to investigate biological properties on anti-herpes simplex viruses, phytochemical compounds and antioxidant activities of extracts obtained from riceberry and black rice. The highest nontoxic concentration of extracts were used for treatment with both type of herpes simplex virus (HSV). The results showed that aqueous extract of black rice showed the highest anti-HSV-2 when treated during viral attachment with inhibition of 80.45 ± 1.28% and ED₅₀ = 1,957.55 ± 13.73 µg/ml. Ethanolic extract of black rice inhibited HSV-2 after viral attachment with 54.26 ± 1.06% and ED₅₀ = 2,492.89 ± 109.72 µg/ml. Moreover, cytotoxicity of rice extracts were determined on Caco-2 colon cancer cells. It found that the extract of riceberry showed the most toxicity on Caco-2 cells. The aqueous extract of riceberry showed high antioxidant activity when measurement by both the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. In addition, the aqueous extract of black rice, contained the highest amount of phenolic compound and the level of total anthocyanin content was the highest in ethanolic extract of black rice.