RSUSSH 2020

IN20-234 Screening and Identification of Rare Actinomycetes Isolated from Soil in Pho Hin Dad Waterfall (Namtok Sam Lan National Park, Saraburi Province) for Antimicrobial Activities

Presenter: Nantawan Niemhom
King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand

Abstract

          Green nanoscale zero-valent iron particles represent a new tool of treatment environmental pollution that could provide cost-effective, high surface energy, eco-friendly and high reactive activity to some of the most challenging environmental cleanup problems. In this study, nanoscale zero-valent iron particles (nZVI) were synthesized from weed extracts. Twelve kinds of weed were studied to compare which one is the most suitable for synthesis of nZVI. From the results, Euphorbia hirta possesses the highest phenolic content (3.28 mg GAE/g) and reducing power (586.34 mg/g). nZVI was successfully synthesized by using Euphorbia hirta extract. The synthesized nZVI was used for the decolorization of azo dyes. With an initial dye concentration of 100 mg/L and nZVI dose of 1g/l, the removal efficiency reached over 99% within 60 min.

Keywords: Rare actinomycetes; Antimicrobial activity; Identification; 16S rRNA; Pho Hin Dad Waterfall

Citation format:

Niemhom, N., & Thawai, C.. (2020). Screening and Identification of Rare Actinomycetes Isolated from Soil in Pho Hin Dad Waterfall (Namtok Sam Lan National Park, Saraburi Province) for Antimicrobial Activities. Proceeding in RSU International Research Conference, May 1, 2020. Pathum Thani, Thailand.

QUESTIONS & ANSWERS

Asst. Prof. Dr. Thanet Sophonnithiprasert (Chairperson)

Your work is very interesting research. Interestingly, the bootstrap value in the result of phylogram is showed that the isolate P15 may be novel strain of Actinomadura. I have some questions on the result of antimicrobial activities which showed in your slide presentation (slide No.10). The graph show number of selected isolates able to inhibit microorganisms by referred the result of disk diffusion assay. What is the average size of clear zone for positive results which showed in this graph? All of three isolates have similar efficiency of antimicrobial activity, or not? and What is the concentration of extract used in disk diffusion assay? Thanks in advance for your response.

Nantawan Niemhom (Presenter)

Thank you for your comment and question. I am sorry for the absent data.

For antimicrobial activities, three isolates have similar efficiency of antimicrobial activity. Clear zone sizes are 8-13 mm. The concentration of extracts is 50 mg/ml.

Asst. Prof. Dr. Thanet Sophonnithiprasert (Chairperson)

Thank you for your response.

Nanthaphong Khamthong (Participant)

Thank you for you presentation.

1. Do the authors plan to carry out a broth microdilution study of those actinomycetes extracts? 

2. Compared to a common fermentation of endophytic fungi, how do the authors culture the actinomycetes? Are there any difficulties or special processes culturing the actinomycetes? 

Nantawan Niemhom (Presenter)

Thank you for your questions.

1. I plan to test MIC and MBC in the future.

2. For fermentation, the actinomycetes were cultured in yeast extract-malt extract (ISP2) broth in a rotary shaker at 200 rpm, 30 °C for 14 days.