Tests on repellents were typically performed on shaven animals, such as rabbits, dogs, guinea pigs, chicks, and sheep, as alternative subjects to humans. However, these techniques often raise queries concerning the ethical treatment of animals and human values or practical aspects, which sometimes misinterpret the results of repellent tests. In this study, we developed a test method and improved the evaluation of mosquito repellency time. This method was designed to reduce the risk of contamination with repellents, and for evaluation of the correct effective dose and protection time. DEET (N,N-diethyl-meta-toluamide) was used as a gold standard repellent for testing the equipment for five different concentrations. The method was used to impregnate the net, size (5×5) 25cm²with five different concentrations and placed on the cups for testing the protection time. The protection time was recorded for 5 minutes after 30 minutes of the exposure. The results demonstrate that DEET with 2.5% concentration provided 3 hours of protection while the remaining concentrations 0.75%, 0.50%, 0.25% and 0.01% can protect, 120, 90, 60 and 30 min respectively. The data was analyzed by one-way analysis of variance (ANOVA) and Duncan’s multiple comparison by SPSS for Windows. The effective dose ED₅₀ of DEET was 0.39% which was calculated by Plot (log dose and % of inhibition).This method indicates that in future this innovative testing method can not only be used for repellent testing, but also be used for insecticide.