RSUSSH 2020
IN20-110 Extraction, Isolation and Biological activities of Phanera nakhonphanomensis
Presenter: Wittawat Phonjaroen
Lampang Rajabhat University, Thailand
Abstract
Five known compounds; (1) palmitic acid, (2) lupeol, (3) gallic acid, (4) β-sitosterol-3-O-tetra-O-acetyl-β-D-mannopyranose, and (5) methyl gallate, were isolated from the liana and leaves crude extracts of Phanera nakhonphanomensis. Their structures were elucidated by IR, NMR, and MS data. Gallic acid showed significant cytotoxic activities on against P-388, HT 29, A 549, and CL human cancer cell lines using SRB method with ED50 values of 2.57, 16.27, 11.42, and 12.23 µg/mL, respectively. The Anti-HIV-1RT activity of gallic acid, ethyl acetate extract, and methanol extract showed strong percentage inhibitory values of 91.50%, 92.08%, and 97.63%, respectively.
Citation format:
Phonjaroen, W., Pompimon, W., Udomputtimekakul, P., Nantasaen, N., Tualamoon, J., & Baison, W.. (2020). Extraction, Isolation and Biological activities of Phanera nakhonphanomensis. Proceeding in RSU International Research Conference, May 1, 2020. Pathum Thani, Thailand.QUESTIONS & ANSWERS
1. The main ideal that you would like to isolate compound from the liana and leaves crude extracts of Phanera nakhonphanomensis.
2. What is the application from this research
(1) In this research, the phytochemical investigation of the mixed liana and leaves P. nakhonphanomensis are studied because no research has been found on the composition of compounds in the part of liana and leaves. In the previous work, they discussed relatively good antioxidant activity.
(2) The application of this research is the composition of some bioactive compounds that can be used as a prototype for medicine.
1. Apart from NMR spectroscopic data, how do the author identify and comfirm the stereochemistry of β-sitosterol-3-O-tetra-O-acetyl-β-D-mannopyranose?
2. Only gallic acid was tested against cancer cell lines and HIV; how about the other isolated compounds?
Thank you.
(1) Confirmed using references from Tania Peshin and H. K. Kar research in 2017 that found the same compound, thus helping to support and assist in this confirmation and for the principles of the pure compound to find the melting point and another thing that helps to confirm is the IR spectroscopy method.
(2) Due to the principles of the selection of compounds tested for cancer cell lines and HIV, it has been determined from a crude extract that has an inhibitory effect in the above tests, and known compounds which has test results about the compounds that we discovered in this research.
Thank you.